CTAB Extraction Buffer, 125 mL -from Cepham Life Sciences

Cetyltrimethylammonium bromide (CTAB) is a cationic surfactant, commonly used in the preparation and purification of genomic DNA from different samples, particularly plants. Extracting DNA from plant tissues can be very challenging, primarily due to the presence of a rigid cell wall surrounding the plant cells. Polysaccharides and polyphenols are two classes of plant biomolecules that vary widely between plant species and are very problematic when extracting DNA. Also, the presence of polysaccharides and polyphenols in a DNA preparation can inhibit the use of polymerase chain reaction (PCR) and other downstream applications. Our CTAB Extraction Buffer effectively removes polysaccharides and polyphenols with the use of Polyvinylpyrrolidone, a polyphenol binding agent. CTAB protocols typically require the homogenization of plant samples in CTAB Extraction Buffer prior to centrifugation to pellet the debris and polysaccharides. Since the DNA extract obtained with this method contains both DNA and RNA, RNA can be easily removed by the addition of RNase A. The DNA is precipitated and washed in organic solvents before re-dissolving in aqueous solution. Our CTAB Extraction Buffer contains 2 % CTAB, 20 mM EDTA·Na2·2H2O, 1.4 M NaCl and 100 mM Tris, pH 8. For research use only. Not for human or animal clinical/therapeutic use. Please follow all safety instructions.

• Contains 2% CTAB, 20 mM EDTA Na2·2H2O, 1.4 M NaCl, & 100 mM Tris
• pH 8
• Ready-to-use solution provides savings in labor, raw materials, and waste disposal
• Each production lot is manufactured in our USA laboratory following stringent Good Manufacturing Practices and rigorous quality control tests
• Product is guaranteed to perform as per its SOP and is backed with a money back guarantee